paa hydrogels Search Results


cell culture vessels coated with polyacrylamide (paa) hydrogel  (Matrigen Life Technologies)
90
Matrigen Life Technologies cell culture vessels coated with polyacrylamide (paa) hydrogel
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Cell Culture Vessels Coated With Polyacrylamide (Paa) Hydrogel, supplied by Matrigen Life Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell culture vessels coated with polyacrylamide (paa) hydrogel/product/Matrigen Life Technologies
Average 90 stars, based on 1 article reviews
cell culture vessels coated with polyacrylamide (paa) hydrogel - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
nps-p-paa hydrogel  (Dawley Inc)
90
Dawley Inc nps-p-paa hydrogel
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Nps P Paa Hydrogel, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nps-p-paa hydrogel/product/Dawley Inc
Average 90 stars, based on 1 article reviews
nps-p-paa hydrogel - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
dry polyacrylic acid (paa)-chitosan (chi)-ala interpenetrating network hydrogel (paca) patch  (CH Instruments)
90
CH Instruments dry polyacrylic acid (paa)-chitosan (chi)-ala interpenetrating network hydrogel (paca) patch
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Dry Polyacrylic Acid (Paa) Chitosan (Chi) Ala Interpenetrating Network Hydrogel (Paca) Patch, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dry polyacrylic acid (paa)-chitosan (chi)-ala interpenetrating network hydrogel (paca) patch/product/CH Instruments
Average 90 stars, based on 1 article reviews
dry polyacrylic acid (paa)-chitosan (chi)-ala interpenetrating network hydrogel (paca) patch - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
f3-targeted paa hydrogel nanocarrier f3nc  (NanoCarrier Co)
90
NanoCarrier Co f3-targeted paa hydrogel nanocarrier f3nc
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
F3 Targeted Paa Hydrogel Nanocarrier F3nc, supplied by NanoCarrier Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/f3-targeted paa hydrogel nanocarrier f3nc/product/NanoCarrier Co
Average 90 stars, based on 1 article reviews
f3-targeted paa hydrogel nanocarrier f3nc - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
pva/paa-hydrogele  (Brinkmann Instruments)
90
Brinkmann Instruments pva/paa-hydrogele
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Pva/Paa Hydrogele, supplied by Brinkmann Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pva/paa-hydrogele/product/Brinkmann Instruments
Average 90 stars, based on 1 article reviews
pva/paa-hydrogele - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
2d polyacrylamide (paa) hydrogels  (Matrigen Life Technologies)
90
Matrigen Life Technologies 2d polyacrylamide (paa) hydrogels
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
2d Polyacrylamide (Paa) Hydrogels, supplied by Matrigen Life Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2d polyacrylamide (paa) hydrogels/product/Matrigen Life Technologies
Average 90 stars, based on 1 article reviews
2d polyacrylamide (paa) hydrogels - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
injectable poly(glycerol sebacate)-co-poly(ethylene glycol)/polyacrylic acid (pegs/paa) hydrogel  (Mimetics)
90
Mimetics injectable poly(glycerol sebacate)-co-poly(ethylene glycol)/polyacrylic acid (pegs/paa) hydrogel
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Injectable Poly(Glycerol Sebacate) Co Poly(Ethylene Glycol)/Polyacrylic Acid (Pegs/Paa) Hydrogel, supplied by Mimetics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/injectable poly(glycerol sebacate)-co-poly(ethylene glycol)/polyacrylic acid (pegs/paa) hydrogel/product/Mimetics
Average 90 stars, based on 1 article reviews
injectable poly(glycerol sebacate)-co-poly(ethylene glycol)/polyacrylic acid (pegs/paa) hydrogel - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
sterile hydrogel pieces m1-m6 (chi/paa/pam)a  (CH Instruments)
90
CH Instruments sterile hydrogel pieces m1-m6 (chi/paa/pam)a
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Sterile Hydrogel Pieces M1 M6 (Chi/Paa/Pam)A, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sterile hydrogel pieces m1-m6 (chi/paa/pam)a/product/CH Instruments
Average 90 stars, based on 1 article reviews
sterile hydrogel pieces m1-m6 (chi/paa/pam)a - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
paa hydrogel  (Abaqus Inc)
90
Abaqus Inc paa hydrogel
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Paa Hydrogel, supplied by Abaqus Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/paa hydrogel/product/Abaqus Inc
Average 90 stars, based on 1 article reviews
paa hydrogel - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
gqd-paa hybrid hydrogel  (Shivam Industries)
90
Shivam Industries gqd-paa hybrid hydrogel
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Gqd Paa Hybrid Hydrogel, supplied by Shivam Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gqd-paa hybrid hydrogel/product/Shivam Industries
Average 90 stars, based on 1 article reviews
gqd-paa hybrid hydrogel - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
paa hydrogels  (Loctite)
90
Loctite paa hydrogels
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
Paa Hydrogels, supplied by Loctite, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/paa hydrogels/product/Loctite
Average 90 stars, based on 1 article reviews
paa hydrogels - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
m4(chi/paa/ pam)a hydrogel  (CH Instruments)
90
CH Instruments m4(chi/paa/ pam)a hydrogel
(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. <t>PAA</t> = <t>polyacrylamide.</t> (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm
M4(Chi/Paa/ Pam)A Hydrogel, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m4(chi/paa/ pam)a hydrogel/product/CH Instruments
Average 90 stars, based on 1 article reviews
m4(chi/paa/ pam)a hydrogel - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


(A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. PAA = polyacrylamide. (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm

Journal: bioRxiv

Article Title: Mechanical environment afforded by engineered polymer hydrogel critically regulates survival of neural stem cells transplanted in the injured spinal cord via Piezo1-mediated mechanotransduction

doi: 10.1101/2025.04.15.648586

Figure Lengend Snippet: (A) A diagram depicting the in vitro culture system providing polymer matrix with varying degree of mechanical stiffness. PAA = polyacrylamide. (B) Representative images of NSCs obtained during adhesion assay. NSCs expressing GFP were grown on substrates with mechanical stiffness ranging from 25 kPa to 0.2 kPa. Upper panel: Phase-contrast images. Middle panel: NSCs were visualized using GFP fluorescence. Boxed regions show magnified cell morphologies below. Scale bars = 20 μm. (C-E) Quantitative graphs comparing the number of cells adhered (C), areas of cell spreading (D), and the perimeter of cell boundary (E). Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars represent SEM. ** and *** indicates p < 0.05 and p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. ( F-G) Representative images of NSCs for cell survival assay. NSCs were cultured on hydrogel substrates ranging from 25 kPa to 0.2 kPa stiffness for 24 hrs. Subsequently, cell survival assay was performed. Live cells are labeled by Calcein-AM (green), and dead cells are labeled by Ethidium Homodimer-1 (red). Quantitative graph depicting the percentage of dead cells (G) . Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Error bars indicate SEM. Scale bars = 100 μm. (H-I) Representative images of phospho-ERK immunostaining (H) . Arrows indicate GFP positive cells colocalized with phospho-ERK (pERK, magenta) expression. The total number of NSCs was divided by pERK positive cells (I) . Error bars represent SEM. ** indicates p < 0.01 by unpaired t-test. Each dot represents an independent culture replicate, with each replicate being an average value from three coverslips. Scale bar = 50 μm. ( J-K) Representative images of NSCs for cell survival assay with sodium arsenite treatment. Sodium arsenite (NaAsO 2 ) was used to induce cellular stress for 24 h (J) . Quantitative graph of percentage of dead cells (K) . N = 6 and 4 for 25 kPa and 0.2 kPa groups, respectively. Error bars represent SEM. * indicates p < 0.05 by unpaired t-test. Error bars represent the SEM. Scale bars = 100 μm

Article Snippet: Cell culture vessels coated with polyacrylamide (PAA) hydrogel, featuring different elastic modulus levels, were purchased from Matrigen ( http://matrigene.com ).

Techniques: In Vitro, Polymer, Cell Adhesion Assay, Expressing, Fluorescence, Clonogenic Cell Survival Assay, Cell Culture, Labeling, Immunostaining